Multicellular Decision Making

How do cells coordinate their behaviors? Cells work together and make group decisions in systems ranging from bacterial biofilms to healing wounds to neuronal networks to cancer tumors. However, our understanding of how these collectives coordinate themselves is in its infancy when compared to what we know about how single cells function as individuals. This knowledge gap exists because of the technological challenges presented by connecting microscale events happening on a timescale of seconds inside cells to the macroscale events occurring over hours in collectives. Our lab addresses these challenges through creating and applying new methods to observe and control these behaviors. These new techniques enable us to develop a predictive understanding of how processes inside a single cell shape collectives, permitting us to engineer these group-wide behaviors for our benefit.

Tools

Tools we use and optimize include (but aren't limited to):

  • genetically-encoded fluorescent biosensors
  • widefield fluorescence microscopy
  • darkfield microscopy
  • confocal microscopy
  • optogenetics
  • microfluidics
  • computational modeling

Aggregating Fluorescent Slime Mold

Collective Model Systems

We work with a wide range of model systems to help us in our long term goal of identifying general principles biological systems implement to coordinate collectives.

Cellular Slime Mold

Dictyostelium discoideum, a cellular slime mold also known as the social amoeba, exists as a solitary amoeba when food is available. However, when they run out of food and starve, the amoebas begin to signal one another with waves of signaling molecules that form spirals and targets. As starvation continues, the cells begin chemotaxing toward the perceived center of these waves to aggregate together and form a multicellular fruiting body structure to be more easily transported to a new area with more food. Dictyostelium is a fantastic model system for studying cellular collective behavior because it is one of the few systems where we have the ability to simultaneously control collective signaling at the level of single cells and observe an entire collective and its signaling responses and behaviors simultaneously. To allow for this level of control, we have developed a toolbox of microfluidic, optical, and genetic tools. Working with these techniques we can address questions such as:

  • What are the essential signaling dynamics for initiating and maintaining collective behavior?
  • How do cells self-organize into patterns?
  • How do cells specify their fates and exploit their individuality in collectives?
  • How robust and reproducible are these collective behaviors?
  • How can we re-program cellular collectives?
Fibroblast Wound Healing

Collective signaling and cell migration during wound healing allow us to explore how cells work together as a group to repair damage and navigate an unexpected situation. We are currently combining our expertise in visualizing signaling dynamics with model wound healing systems to answer questions such as:

  • What signaling responses are responsible for wound healing?
  • Can we control and manipulate the wound healing response?
Bacterial Biofilms

Bacterial biofilms offer us an opportunity to understand how different species can work together and exploit their native communication strategies to cooperate for the benefit of the collective. Many of the questions in biofilms are similar to those we ask in Dictyostelium, but require new techniques and approaches to answer.

Image Credits: Photos adapted from Höfer et al. Dictyostelium discoideum: Cellular Self-Organization in an Excitable Biological Medium (1995) and Owen Gilbert